Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. TRIZOL Reagent (U.S.Patent No.34994) is a ready-to-use reagent for the isolation of total RNA from cells and tissues. This method is particularly . Synony3-(4- Dichlorophenyl)-6-fluoro-2-(1H-4- trizol -1-yl)quinazolin-4(3H)-one, Castellan.

Empirical Formula (Hill Notation): C16H8Cl2FN5O.

The mix is then vortexed vigorously for about min, centrifuged briefly, and incubated at room . Unbiased reviews by scientists available at Biocompare. Isolation of RNA from Small Quantities of Tissue Samples. Procedure for use when DNA is not neede . Sample volume shoµld not exceed of the volume of Trizol. Allow minute incubation at room temp.

Please do not make copies of or distribute this protocol.

Required reagents: DEPC-treated water (Ambion). Equipment and supplies: Refrigerated centrifuge. RNA extraction with Trizol. For Whole Tissues: – Isolate tissue and snap freeze in liquid nitrogen.

Recommended: Freeze in Trizol at least hr . Transfer 1ml each into two fresh 1. TRIzol Reagent (Invitrogen). We also demonstrate using polyacrylamide gel electrophoresis ( PAGE) and Western blot analysis that the proteins, representing both a wide molecular . TRIZOL has demonstrated stability of months when stored at room temperature. The reagent, a mono-phasic . Analytical Biochemistry 162: 156- 159.

Commercially available from many . RNA Extraction with TRIZOL. Wear gloves when handling trizol , work RNase- free! Aspirate medium from cells grown in dishes.

In the fume hoo add 4ml of Trizol to cells on plastic of 10cm plate.

Cap sample tubes securely. Shake tubes vigorously by hand for seconds and incubate them at . Close tubes securely (BE CAREFUL, THE REAGENT CONTAINS PHENOL). Chloroform EtOH diluted with DEPC H2O.