High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Type, Vender, Cost per unit, Product Information, Image. Polymerase offers extreme performance for all major PCR applications. Taq DNA polymerase , NEB, $0. Hot Start PCR – High-throughput PCR – High Fidelity PCR – Long-range PCR, – Standalone enzyme – Standalone enzyme with green buffer – Master mix – Master mix with green . As a rule of thumb, I wanted to go with thermo- stable polymerase having a fold less error rate compared to Taq polymerase.
Phusion High Fidelity DNA polymerase , NEB, $0. An added advantage was that it comes with GC enhancer buffer if the . Xia Y(1), Chu W(1), Qi Q(1), Xun L(2). Author information: (1)State Key Laboratory of Microbial Technology, . Component, µl Reaction, µl Reaction, Final Concentration. Nuclease-free water, to µl, to µl. M Forward Primer, µl, 2. M Reverse Primer, µl, 2. Template DNA , variable, variable . This in shorter extension times, stronger bands and . In the laboratory setting, Pfu is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new . This enzyme keeps significant activity after exposure to 99°C or repeated exposure to 98°C with more processivity and extention rate than Pfu DNA polymerase.
Adavatages: Ultrapure recombinant . Pyrococcus-like enzyme with a processivity-enhancing domain. Median bypass probabilities from four independent reactions are shown. See Supplementary Table for more statistics. First left lane, ss DNA ladder.
Site-directed mutagenesis is widely used in the study of gene and protein functions. Due to this unique fusion technique, Phusion. DNA Polymerases generate PCR products with accuracy and speed unattainable with a single enzyme, even on the most difficult templates.
View Detail Add to Order. Here we present a fast and simple protocol to amplify DNA directly from disrupted formalin -fixed paraffin-embedded (FFPE) tissues without the need for time consuming and. The enzyme has a molecular weight of approximately 90daltons as estimated from the predicted amino acid sequence and . PCR reactions should be set up on ice. Prepare a master mix for the appropriate number of samples to be amplified.
This is a fusion protein of Pfu DNA polymerase and a DNA binding protein, Sso7 from S. Sso7d has strong affinity to DNA, and it retains the fused Pfu DNA . They offer a combination of characteristics that no other enzyme can match.